The default is to have FlowJo determine the optimal limits. You can manually set the lower and upper limits on the Y axis. The Y-axis for histograms is the number of cells/events falling within each “bin” of the histogram there are 256 bins for each histogram which correspond to 256 pixels of display space. This can be done in the FlowJo Layout Editor.įluorescence intensity is displayed on the X-axis (divided into 256 bins) and the count of events in each fluorescence channel is displayed on the Y-axis. Normalizing plots to unit area is good for comparison purposes. It is important to show the frequency or count of the population in a table or on the plot. *Remember that peak height is a function of the CV (spread) and number of events, so histogram displays can be misleading. (Open Options in the Graph Window and uncheck smooth to show unsmoothed). Below are the same data-set shown with and without smoothing. The histograms can be smoothed (by default) or unsmoothed this option has little effect when more than a few thousand events are in the gate.
Histogram Options: Smoothing, Scaling and Color See the sections below for more information about histogram options and gating tools. In contrast to dot plots or other bivariate displays, there are different types of options and gating tools for histograms. 7-AAD) was used during staining, you can quickly evaluate whether you have high or low numbers of live cells (live cells will be largely 7-AAD negative). This can be helpful to quickly analyze whether your samples have normal, uniform, bimodal, or other type of distribution.
Histograms display a frequency distribution of the data versus fluorescence intensity or some other parameter (e.g.
Use histograms to view frequency distribution of your flow data, one parameter at a time. To view your plot as a histogram, simply click the drop-down menu on the left side of the Graph Window and select “Histogram” from the menu. FlowJo v10 makes it easy to convert bivariate dot plots to univariate histograms with a click of a button!
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